Searching for genetic factors involved in hybrid lethality in Nicotiana; Possible use of a cultured cell line of a hybrid
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- Mino Masanobu
- Graduate School of Agriculture, Kyoto Prefectural University
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- Kubota Mami
- Graduate School of Agriculture, Kyoto Prefectural University
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- Yamaguchi Yuki
- Graduate School of Agriculture, Kyoto Prefectural University
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- Kawamori Tetsu
- Graduate School of Agriculture, Kyoto Prefectural University
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- Sato Kenji
- Department of Food Science and Nutritional Health, Kyoto Prefectural University
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- Misaka Yuko
- Graduate School of Agriculture, Kyoto Prefectural University
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- Tanaka Yoshikazu
- The Wakasa-wan Energy Research Center
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- Inoue Masayoshi
- Graduate School of Agriculture, Kyoto Prefectural University
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Abstract
The cell line GTH4 generated from the F1 hybrid of Nicotiana gossei×N. tabacum reveals lethality at the seedling stage. The cells proliferated at 37°C but died at 26°C. GTH4S, a cell line derived from GTH4 that does not die at 26°C, was established. Differences in the genes and proteins expressed in the two cell lines were analyzed. GTH4 expressed the gene encoding ubiquitin-conjugating enzyme (E2) which was not expressed in GTH4S. PCR targeting this sequence indicated that GTH4S is missing a segment of DNA containing the E2 locus, suggesting that GTH4S is a deletion mutant of GTH4. An analysis of proteins using anion-exchange HPLC and SDS-PAGE revealed GTH4 to contain protein species not found in GTH4S. The genes and/or proteins involved in the expression of hybrid lethality can be analyzed by comparing these two cell lines.
Journal
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- Plant Biotechnology
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Plant Biotechnology 23 (5), 487-492, 2006
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304054656
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- NII Article ID
- 10021909543
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 8571162
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed