Comparative analysis of expression profiles of counterpart gene sets between Brassica rapa and Arabidopsis thaliana during fungal pathogen Colletotrichum higginsianum infection
To perform comparative sequence and transcriptome analyses between <i>Brassica rapa</i> and <i>Arabidopsis thaliana</i>, we prepared a <i>B. rapa</i> cDNA microarray using 1,820 (ca. 2 K) cDNA clones selected from 2,166 non-redundant sequences of cDNA library of Chinese cabbage. The gene expression during infection with fungal pathogen <i>Colletotrichum higginsianum</i> and treatments with signaling molecules was analyzed using 2 K <i>B. rapa</i> and 1.2 K <i>Arabidopsis</i> cDNA microarrays. In <i>B. rapa</i>, the results suggested a large correlation coefficient between compatible pathogen <i>C. higginsianum</i>-infection and the treatment with salicylic acid, methyl jasmonate, or ethephon. The expression profiles of 145 counterpart gene sets between the <i>B. rapa</i> and <i>Arabidopsis</i> were distributed in the self-organizing map analysis. The 28% of them indicated similarities in the two species transcriptome. These expressed sequence tag (EST) and microarray data should provide a valuable resource for functional genomics on the crops.
- Plant biotechnology
Plant biotechnology 23(5), 503-508, 2006-12-01
Japanese Society for Plant Cell and Molecular Biology