Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter
The fluorescent signal of GFP fused to CIG-B, which is one of the three <i>DREB1</i>/<i>CBF</i> (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of <i>GFP</i> driven by <i>CIG-B</i> promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in <i>CIG-B</i> promoter could be responsible for nuclear targeting and low temperature perception, respectively.
- Plant biotechnology
Plant biotechnology 23(5), 515-518, 2006-12-01
Japanese Society for Plant Cell and Molecular Biology