Random amplified polymorphic DNA analysis to distinguish Brugmansia suaveolens, B. candida and B. versicolor

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DNA isolation followed by random amplified polymorphic DNA (RAPD) analysis was assessed as a possible method to distinguish three Indonesia species of <i>Brugmansia</i> because these species cannot be distinguished by metabolite analysis or morphological observation when they are not flowering. The DNA fragments were obtained by PCR, using random primers of isolated DNA, and the RAPD analysis showed that each species had different DNA fragment patterns, thus allowing each species to be individually distinguishable. The DNA patterns of each species remained the same, regardless of the samples region in Indonesia.

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  • Plant biotechnology  

    Plant biotechnology 23(5), 519-520, 2006-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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