Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars
The purpose of this study was to develop a reproducible efficient procedure for the transformation of Javanica rice cultivars from Indonesia. Five rice cultivars cultivated in Indonesia now were examined for their capacity on the callus growth, plant regeneration and transformation. Their potential was affected by genotype and medium. Regarding the quality of callus, type I calli produced higher plant regeneration frequency than type II calli. So type I calli were inoculated with <i>A. tumefaciens</i> harboring binary plasmid pAFT14, which had a hygromycin resistance (<i>hpt</i>) gene and a <i>gus</i> gene. In this study, we examined two media for two steps in the tissue culture process for transformation, i.e. callus inducing and plant regeneration. The results show that C-modified medium was the suitable media for callus induction in the most Javanica rice cultivars. <i>Agrobacterium</i>-mediated transformation system has been extended to five Javanica rice cultivars. Among them, Rojolele consistently gave the best performance.
- Plant biotechnology
Plant biotechnology 23(5), 521-524, 2006-12-01
Japanese Society for Plant Cell and Molecular Biology