Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives

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Protoplast culture and shoot regeneration from protoplast-derived calli were compared among different organs of Solanum integrifolium and three of its wild relatives (S. abutiloides, S. scabrum, and S. toxicarium). Leaves, cotyledons, and hypocotyls were used as sources of protoplast preparation. After one month of culture, a high frequency of visible colony formation was obtained from cotyledon protoplasts of S. integrifolium and S. scabrum, hypocotyl protoplasts of S. integrifolium and S. abutiloides, and leaf protoplasts of S. integrifolium and S. toxicarium. In addition, when the primary culture was started at a density of 2.5−5×104 protoplasts ml−1, the highest frequency of colony formation was obtained. Moreover, when the colonies were subcultured for 7 days on solid callus-proliferation medium before being transferred to shoot-induction medium, the plant regeneration frequency improved to between 91.8 and 98.8%.

Protoplast culture and shoot regeneration from protoplast-derived calli were compared among different organs of <i>Solanum integrifolium</i> and three of its wild relatives (<i>S. abutiloides</i>, <i>S. scabrum</i>, and <i>S. toxicarium</i>). Leaves, cotyledons, and hypocotyls were used as sources of protoplast preparation. After one month of culture, a high frequency of visible colony formation was obtained from cotyledon protoplasts of <i>S. integrifolium</i> and <i>S. scabrum</i>, hypocotyl protoplasts of <i>S. integrifolium</i> and <i>S. abutiloides</i>, and leaf protoplasts of <i>S. integrifolium</i> and <i>S. toxicarium</i>. In addition, when the primary culture was started at a density of 2.5−5×10<sup>4</sup> protoplasts ml<sup>−1</sup>, the highest frequency of colony formation was obtained. Moreover, when the colonies were subcultured for 7 days on solid callus-proliferation medium before being transferred to shoot-induction medium, the plant regeneration frequency improved to between 91.8 and 98.8%.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 23(5), 525-529, 2006-12-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10021909694
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8571227
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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