Identification of hemizygous and homozygous transgenic rice plants in T_1 generation by DNA blot analysis

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Southern hybridization-based zygosity analysis was done in a transgenic rice plant (<i>Oryza sativa</i> L. cv Pusa Basmati 1) generated by <i>Agrobacterium</i>-mediated transformation with a rice chitinase (<i>chi</i>11) gene. A T<sub>0</sub> plant with two unlinked T-DNA insertions (A and A′), was chosen for the application of Southern hybridization analysis to study genetic separation of the two loci by segregation and to identify the homozygous and hemizygous plants in T<sub>1</sub>- generation. The T<sub>1</sub> plants showed differences in band intensities that reflected the homozygous and hemizygous status of each of the two integration events. The predictions of zygosity of T<sub>1</sub> plants were confirmed by analyzing segregation in T<sub>2</sub> plants. Southern hybridization analysis is demonstrated as a simple and effective method to distinguish hemizygous and homozygous plants in the T<sub>1</sub> generation itself.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(5), 531-534, 2006-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021909716
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8571260
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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