The tobacco CHN50 matrix attachment region enhances transformation frequency
The nuclear matrix attachment region (MAR) located upstream from the tobacco gene <i>CHN50</i>, designated CHN50MAR, consists of two MAR elements: S/M I and S/M II. In a direct gene transfer system using tobacco BY-2 cells, CHN50MAR produced a several-fold increase in colony formation of hygromycin-resistant (hyg<sup>R</sup>) cells when arranged adjacent to both the 5′ and 3′ termini of a hygromycin-selective marker gene. S/M I and S/M II enhanced hyg<sup>R</sup> colony formation to the same degree as CHN50MAR, suggesting that these MAR elements are functionally redundant for enhancing transformation. The presence of the marker genes in the hyg<sup>R</sup> cells was confirmed using the polymerase chain reaction. These results imply that CHN50MAR and its MAR elements are available as transformation enhancers in direct gene transfer systems for plants.
- Plant biotechnology
Plant biotechnology 23(5), 535-538, 2006-12-01
Japanese Society for Plant Cell and Molecular Biology