The tobacco CHN50 matrix attachment region enhances transformation frequency

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The nuclear matrix attachment region (MAR) located upstream from the tobacco gene <i>CHN50</i>, designated CHN50MAR, consists of two MAR elements: S/M I and S/M II. In a direct gene transfer system using tobacco BY-2 cells, CHN50MAR produced a several-fold increase in colony formation of hygromycin-resistant (hyg<sup>R</sup>) cells when arranged adjacent to both the 5′ and 3′ termini of a hygromycin-selective marker gene. S/M I and S/M II enhanced hyg<sup>R</sup> colony formation to the same degree as CHN50MAR, suggesting that these MAR elements are functionally redundant for enhancing transformation. The presence of the marker genes in the hyg<sup>R</sup> cells was confirmed using the polymerase chain reaction. These results imply that CHN50MAR and its MAR elements are available as transformation enhancers in direct gene transfer systems for plants.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(5), 535-538, 2006-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021909731
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8571266
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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