Molecular and genetic characterization of transgenic tomato expressing 3-hydroxy-3-methylglutaryl coenzyme A reductase

この論文にアクセスする

この論文をさがす

著者

抄録

Mevalonic acid (MVA) pathway, in parallel with methylerythritol phosphate (MEP) pathway, produces precursor metabolites for isoprenoids, and affect fruit development in plants. 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is supposed to be a key enzyme in the MVA pathway. To understand the role of HMGR in fruit development, we previously generated transgenic tomato (<i>Solanum lycopersicum</i>) expressing a melon <i>HMGR</i> gene (<i>CmHMGR</i>) and observed increased fruit size in these plants. To further examine this effect, we performed molecular and genetic characterization of the transgenic tomato line in the T<sub>4</sub> generation. The line showed stable expression of <i>CmHMGR</i> mRNA and protein, an effect that could lead to the increase in fruit weight observed, which exceeded 20%. Interestingly, the <i>CmHMGR</i> mRNA was highly expressed during tomato fruit development, whereas expression of the endogenous <i>HMGRs</i> (<i>SlHMGR1</i>, <i>SlHMGR2</i>, and <i>SlHMGR3</i>) was lower than in the wild type, suggesting the presence of a regulatory mechanism at the transcriptional level, as in mammalian systems. A preliminary analysis using cDNA macroarray filters was performed, and genes showing more than 2.5-fold differences in expression between transgenic and wild-type plants were identified. Most of the genes involved in isoprenoid biosynthesis did not show significantly different transcription levels, but 121 annotated genes and 152 genes of unknown function were found to be differentially expressed. These results demonstrate that the transgenic tomato line expressing the <i>HMGR</i> gene is genetically stable and could be used as a comprehensive material to elucidate the roles of HMGRs in tomato fruit development.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(1), 107-115, 2007-03-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  36件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021910422
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    8690527
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ