Direct interaction between S-locus receptor kinase and M-locus protein kinase involved in Brassica self-incompatibility signaling

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Many flowering plants possess self-incompatibility (SI) systems to prevent inbreeding. In <i>Brassica</i>, SI recognition is controlled by the multiallelic gene complex (<i>S</i>-haplotypes) at the <i>S</i>-locus, which encodes both the male determinant (<i>S</i>-locus protein 11 (SP11/SCR)) and the female determinant (<i>S</i>-receptor kinase (SRK)). After self-pollination, an <i>S</i>-haplotype specific interaction between pollen-borne SP11 and its cognate stigmatic SRK receptor induces SI signaling in stigma papilla cells, which results in the rejection of the self-pollen. Previous genetic analysis by our group of a self-compatible mutant revealed the involvement of a membrane-anchored cytoplasmic protein kinase, <i>M</i>-locus protein kinase (MLPK), in SI signaling. The plasma membrane localization of MLPK suggests that it functions in the vicinity of SRK, but the physiological relationship between the two proteins remains unknown. In the present study, we looked for a direct interaction between SRK and MLPK using both genetic and biochemical techniques. Although a conventional yeast two-hybrid system and a split-ubiquitin membrane-based yeast two-hybrid system failed to detect a direct interaction between SRK and MLPK, an <i>in vitro</i> phosphorylation assay indicated that the kinase domain of SRK could efficiently phosphorylate MLPK. These results suggest that MLPK could be a direct target of SRK in <i>Brassica</i> SI signaling.

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  • Plant biotechnology  

    Plant biotechnology 24(2), 185-190, 2007-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021910967
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    8690323
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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