Isolation and characterization of novel genes controlled by short-day treatment in Pharbitis nil
The short-day plant <i>Pharbitis</i> [<i>Ipomoea</i>] <i>nil</i> has been a model plant in physiological studies of photoperiodic control of floral initiation. However, molecular mechanisms underlying photoperiodic responses, including the induction of flowering in <i>P. nil</i>, are largely unknown. Here we identified and characterized cDNAs whose expression patterns were different in short- and long-day conditions, based on fluorescent differential display and RNA gel blot analysis, to gain insight into the molecular mechanisms of photoperiodic responses in <i>P. nil</i>. The cDNA clones included genes encoding ADP-glucose pyrophosphorylase, a putative protein kinase, eukaryotic initiation factor eIF-4, a putative aldehyde dehydrogenase, subtilisin-like proteinase, crooked neck (crn)-like protein, and cryptochrome 1. The possible roles of these genes in the photoperiodic response, including the control of flowering time in <i>P. nil</i>, are discussed.
- Plant biotechnology
Plant biotechnology 24(2), 201-207, 2007-03-01
Japanese Society for Plant Cell and Molecular Biology