Jasmonate-induced 23kD protein, JIP-23, is involved in seed development of barley
Access this Article
Search this Article
We previously showed the high expression of a 23 kD protein (P23k) in germinating barley seeds. In this study, we examined whether P23k is involved in supplying sugar to developing barley grains. RT-PCR analysis revealed that, although P23k expression was not detectable in developing grain, the 23 kD jasmonate-induced protein (JIP-23) exhibited a high level of RNA expression. JIP-23 belongs to the same protein family as P23k. Its remarkable expression was specifically confirmed at early development stage when sugar import into the filling grains is active. Furthermore, <i>in situ</i> hybridization and immunohistochemistry showed that JIP-23 is localized to active tissues in sugar import, such as vascular tissues, nucellar projections, endosperm transfer cells, and inter nucellar cells. Taken together, these results suggest that JIP-23 is related to sugar import into filling grain of developing barley seeds. Hence, we propose that jasmonate may regulate the development of barley seed.
- Plant tissue culture letters
Plant tissue culture letters 24(2), 217-220, 2007-03-01
Japanese Society for Plant Cell and Molecular Biology