Cloning and characterization of ACC oxidase genes from tulip

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Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (<i>Tulipa gesneriana</i> L.) by differential screening of the petal cDNA library and were designated as <i>TgACO1</i>, <i>TgACO2</i>, <i>TgACO3</i>, <i>TgACO4</i> and <i>TgACO5</i>. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity (90–94% identity) each other, whereas TgACO5 showed low similarity compared with the other four proteins (50% identity). Phylogenic analysis indicated that <i>TgACO1-4</i> and <i>TgACO5</i> are distant. Genomic analysis of <i>TgACO1-4</i> showed that they are organized into four exons and three introns, whereas <i>TgACO5</i> consists of three exons interrupted by two introns. Real-time RT-PCR analysis of gene expression revealed that <i>TgACO1</i>, <i>TgACO3</i> and <i>TgACO5</i> were expressed in wilting petals, leaves, and stems, respectively, whereas <i>TgACO2</i> and <i>TgACO4</i> were expressed only at basal levels in these tissues. Therefore, tulip ACO genes seem to be regulated differentially among the vegetative tissues and during flower senescence.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(2), 241-246, 2007-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021911208
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8690498
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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