Cloning and characterization of ACC oxidase genes from tulip
Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (<i>Tulipa gesneriana</i> L.) by differential screening of the petal cDNA library and were designated as <i>TgACO1</i>, <i>TgACO2</i>, <i>TgACO3</i>, <i>TgACO4</i> and <i>TgACO5</i>. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity (90–94% identity) each other, whereas TgACO5 showed low similarity compared with the other four proteins (50% identity). Phylogenic analysis indicated that <i>TgACO1-4</i> and <i>TgACO5</i> are distant. Genomic analysis of <i>TgACO1-4</i> showed that they are organized into four exons and three introns, whereas <i>TgACO5</i> consists of three exons interrupted by two introns. Real-time RT-PCR analysis of gene expression revealed that <i>TgACO1</i>, <i>TgACO3</i> and <i>TgACO5</i> were expressed in wilting petals, leaves, and stems, respectively, whereas <i>TgACO2</i> and <i>TgACO4</i> were expressed only at basal levels in these tissues. Therefore, tulip ACO genes seem to be regulated differentially among the vegetative tissues and during flower senescence.
- Plant biotechnology
Plant biotechnology 24(2), 241-246, 2007-03-01
Japanese Society for Plant Cell and Molecular Biology