Cloning and characterization of ACC oxidase genes from tulip

DOI Web Site 参考文献33件
  • Momonoi Kazumi
    Agricultural Experiment Station, Toyama Agricultural Research Center Graduate School of Information Science, Nagoya University
  • Shoji Kazuaki
    Agricultural Experiment Station, Toyama Agricultural Research Center
  • Yoshida Kumi
    Graduate School of Information Science, Nagoya University

この論文をさがす

抄録

Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (Tulipa gesneriana L.) by differential screening of the petal cDNA library and were designated as TgACO1, TgACO2, TgACO3, TgACO4 and TgACO5. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity (90–94% identity) each other, whereas TgACO5 showed low similarity compared with the other four proteins (50% identity). Phylogenic analysis indicated that TgACO1-4 and TgACO5 are distant. Genomic analysis of TgACO1-4 showed that they are organized into four exons and three introns, whereas TgACO5 consists of three exons interrupted by two introns. Real-time RT-PCR analysis of gene expression revealed that TgACO1, TgACO3 and TgACO5 were expressed in wilting petals, leaves, and stems, respectively, whereas TgACO2 and TgACO4 were expressed only at basal levels in these tissues. Therefore, tulip ACO genes seem to be regulated differentially among the vegetative tissues and during flower senescence.

収録刊行物

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ