Pathogenesis-related protein 1 homologue is an antifungal protein in Wasabia japonica leaves and confers resistance to Botrytis cinerea in transgenic tobacco
An antimicrobial protein was purified from leaves of <i>Wasabia japonica</i> L., and designated as WjAMP-2. WjAMP-2 strongly inhibited growth of four phytopathogenic fungi. The deduced amino acid sequence from WjAMP-2 cDNA showed a homology with pathogenesis-related protein 1 (PR-1) from several higher plants. Expression of <i>WjAMP-2</i> was induced by inoculation with fungal pathogens and with treatments by salicylic acid, but not by methyl jasmonate. Overexpression of <i>WjAMP-2</i> in tobacco plants conferred resistance against <i>Botrytis cinerea</i>. These results suggest that WjAMP-2 (PR-1) is an antifungal protein with a defensive role against phytopathogenic fungi in wasabi plants.
- Plant biotechnology
Plant biotechnology 24(2), 247-253, 2007-03-01
Japanese Society for Plant Cell and Molecular Biology