Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis
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<i>Phalaenopsis</i> hybrids constitute a major ornamental crop. An important parent species for many of these hybrids is <i>Phalaenopsis amabilis</i>. We developed a convenient method for the genetic modification of <i>P. amabilis</i> using <i>Agrobacterium tumefaciens</i>. The transformed intact protocorms, which are young orchid seedlings of <i>P. amabilis</i>, regenerated plants under the same conditions that showed the highest frequency of shooting. A kanamycin resistance gene under the control of the 35S promoter can be used as a selective marker. In addition, T-DNA vectors containing the <i>Arabidopsis class</i> 1 <i>KNOX</i> gene, <i>BP</i>/<i>KNAT1</i>, were successfully introduced into protocorms. Shoots were generated with an abnormal leaf shape that was easily distinguished from that of normal shoots, indicating that <i>BP</i>/<i>KNAT1</i> can be used as a visible marker gene. Furthermore, the protocorms transformed with <i>BP</i>/<i>KNAT1</i> produced multiple shoots. Both the presence and expression of the transgene in transformed plants were confirmed by molecular analysis.
- Plant tissue culture letters
Plant tissue culture letters 24(3), 265-272, 2007-06-01
Japanese Society for Plant Cell and Molecular Biology