Identification of tobacco genes encoding proteins possessing removal activity of 5-methylcytosines from intact tobacco DNA

この論文にアクセスする

この論文をさがす

著者

抄録

Cytosines in eukaryotic DNA is often methylated to yield 5-methylcytosines (m<sup>5</sup>C), which play an important role in controlling gene expression. This is referred as DNA methylation, and its status dynamically changes during plant growth by active methylation and demethylation. DNA glycosylases have been known to possess base excision DNA repair activity, and an Arabidopsis enzyme, ROS1 (Repressor of Silencing 1), was recently shown to exhibit m<sup>5</sup>C DNA glycosylase activity. We isolated and characterized four genes encoding ROS1-like proteins from tobacco plants (<i>Nicotiana tabacum</i>), and designated as <i>NtROS1</i>, <i>NtROS2a</i>, <i>NtROS2b</i> and <i>NtROS3</i>, each respectively encoding a polypeptide with 1796, 1673, 1673 and 1662 amino acids. Purified NtROS1 and NtROS2a proteins expressed in Sf9 insect cells clearly exhibited activity of m<sup>5</sup>C removal from tobacco genomic DNA <i>in vitro</i>. GFP fusion assay showed that NtROS1 and NtROS2a were localized in nucleus. Transcripts of <i>NtROS1</i>, <i>NtROS2a</i> and <i>NtROS3</i> were induced by abiotic stresses, including aluminium, salt and reactive oxygen species. These observations suggested that NtROS proteins function in demethylating process of genomic DNA during plant stress response, thereby maintaining the balance of gene expression in combination with DNA methyltransferases.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(3), 339-344, 2007-06-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  29件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021911640
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    8775673
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ