Mobilization of a retrotransposon in 5-azacytidine-treated fungus Fusarium oxysporum
It is well known that DNA methylation is involved in the control of transposable elements in eukaryotic cells. Recent studies indicate that demethylation of DNA in a mutant of <i>Chlamydomonas</i> and <i>Arabidopsis</i> causes transcriptional activation and mobilization of transposons. In this report, transposition of a retrotransposon was investigated in the phytopathogenic fungus <i>Fusarium oxysporum</i> treated with 5-azacytidine (5azaC), a reagent that causes reduction in the DNA methylation level. The results showed elevated transposition frequency in 5azaC-treated isolates when they were incubated for a long time. However, increase of retrotransposon transcripts was not observed, suggesting that the retrotransposon was mobilized by a mechanism other than its transcriptional activation.
- Plant biotechnology
Plant biotechnology 24(3), 345-348, 2007-06-01
Japanese Society for Plant Cell and Molecular Biology