Expression of active enzymes from an inducible tomato-mosaic-virus-based vector in cultured transgenic tobacco BY-2 cells

この論文にアクセスする

この論文をさがす

著者

抄録

Previously, we reported on an inducible viral vector system for foreign protein production in cultured plant cells, in which the transcription of the recombinant viral vector RNA encoding a foreign protein is controlled by an estradiol-inducible promoter. In this study, we used the inducible virus vector system to test the efficiency of a modified tomato mosaic virus (ToMV) encoding the movement protein. The virus inducibly produced a foreign jellyfish green fluorescent protein encoded in the virus in transgenic tobacco BY-2 suspension-cultured cells as efficiently as modified ToMV without the movement protein. We then produced transgenic BY-2 cell lines ER–ToMV–MP–DHFR and ER–ToMV–MP–GUS, which encoded modified ToMV with <i>Escherichia coli</i> dihydrofolate reductase (DHFR) and β-glucuronidase (GUS), respectively. After estradiol was added to the cell culture, DHFR and GUS activity was detected in the ER–ToMV–MP–DHFR and ER–ToMV–MP–GUS cells, respectively. In contrast, no DHFR or GUS activity was detected in untreated transgenic lines. Three days after induction, DHFR accumulation accounted for up to 15% of the total soluble proteins extracted from the cells, indicating that an inducible viral vector is an effective option for efficiently producing active enzymes in cultured plant cells.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(4), 367-373, 2007-09-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  15件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021911763
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    8904828
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ