Expression of Arabidopsis thaliana cytochrome P450 monooxygenase, CYP71A12, in yeast catalyzes the metabolism of herbicide pyrazoxyfen

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In higher plants, cytochrome P450 (P450, CYP) monooxygenases play important roles in the biosynthesis and metabolism of endogenous compounds and/or in the oxidative reactions of xenobiotics, such as herbicides, insecticides, and fungicides. In this study, the novel P450 cDNAs encoding CYP71A12 was isolated from wild-type <i>Arabidopsis thaliana</i> ecotype Ws using an RT-PCR strategy. Northern blot analysis revealed that <i>cyp71A12</i> is expressed specifically in roots but not in flowers, leaves, or stems. To determine the function of CYP71A12, the coding sequences of the corresponding cDNAs were expressed in <i>Saccharomyces cerevisiae</i> AH22 cells. Microsomal fractions expressing CYP71A12 metabolized the herbicide pyrazoxyfen. Liquid chromatography mass spectrometry (LC/MS) analysis indicated that the metabolite of pyrazoxyfen might be <i>N</i>-demethylated at the pyrazole ring and 5-hydroxylated at the dichlorobenzene ring. This is the first report that a pyrazole herbicide is metabolized by a plant P450 species.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(4), 393-396, 2007-09-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021911852
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    8904912
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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