Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction
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<i>Ralstonia solanacearum</i> is a causal agent of bacterial wilt, and the bacterium normally infects through wounds in roots. Since it is not possible to equally and simultaneously inoculate the host plants with bacteria via root inoculation, it has proven difficult to elucidate the molecular events in plants infected with <i>R. solanacearum</i>. To improve the efficiency of inoculation, we inoculated tobacco plants with <i>R. solanacearum</i> by leaf-infiltration. Differential display was carried out to isolate fragments of genes that are regulated in tobacco by virulent strains of <i>R. solanacearum</i> OE1-1 and by the avirulent mutant of <i>R. solanacearum</i>, 31b, which is mutated in the type III secreted PopA protein by transposon insertion. Nineteen <i>R. solanacearum</i>-responsive genes (RsRGs) were successfully isolated using equalized cDNA libraries constructed with water-, <i>R. solanacearum</i> OE1-1- and 31b-infiltrated tobacco leaves. From Northern blot analysis, RsRGs were divided into 3 groups; 1) responsive to both virulent and avirulent bacteria (8 RsRGs), 2) responsive to avirulent bacteria (3 RsRGs), and 3) responsive to virulent bacteria (3 RsRGs). These results suggest that the cDNA pool and method presented in this study provide a valuable resource for functional genomic analysis of <i>R. solanacearum</i>-plant interactions.
- Plant tissue culture letters
Plant tissue culture letters 24(4), 409-416, 2007-09-01
Japanese Society for Plant Cell and Molecular Biology