Jasmonate- and wounding-induced expression of a tobacco gene encoding a BTB domain protein
Nicotine is synthesized in the root of tobacco (<i>Nicotiana tabacum</i>) and transported to aerial parts where this alkaloid functions as an insect repellant. Nicotine biosynthesis is regulated by jasmonate and specific regulatory <i>Nic</i> loci, but molecular components in the jasmonate- or <i>Nic</i>-dependent signaling pathways have not been well characterized. We here identified a tobacco gene encoding a novel protein <i>JEI1</i> (<i><u>J</u>asmonate <u>E</u>arly <u>I</u>nducible 1</i>) containing a BTB domain and a WD40-like repeat. The <i>JEI1</i> expression was regulated by the <i>NIC</i> loci. In the leaf, application of jasmonate or wounding rapidly and transiently induced the <i>JEI1</i> expression, with an induction pattern distinct from a wound-inducible protein kinase <i>WIPK</i>. This tobacco gene might be involved in wound-elicited signaling pathways.
- Plant biotechnology
Plant biotechnology 24(4), 421-424, 2007-09-01
Japanese Society for Plant Cell and Molecular Biology