Evaluation of somatic embryogenesis from immature cotyledons of Japanese soybean cultivars

この論文にアクセスする

この論文をさがす

著者

抄録

To examine the capacity for plant regeneration through somatic embryogenesis, Japanese soybean cultivars were investigated by 3-step-somatic embryogenesis: 1) induction of somatic embryos (SEs) from immature cotyledons, 2) proliferation of SEs in liquid medium and 3) differentiation of SEs into cotyledon-stage embryos. At each step, properties of the cultures varied among cultivars. Notably, significant differences were observed on 1) efficiencies of SE induction, 2) compactness of individual SEs during proliferation, and 3) yields of differentiated embryos (numbers of cotyledon-stage embryos differentiated from 100 mg globular SEs). We found that the compactness of SEs in liquid medium was very important factor for the recovery of well differentiated embryos. In fact, Yuuzuru and Yumeyutaka which indicated high ratios of compact SEs at proliferation step showed high yields of well differentiated embryos. The abilities of these Japanese cultivars to yield cotyledon-stage embryos were superior or comparable to North American cultivar 'Jack', which is used as one of the most preferable genotypes for somatic embryogenesis, indicating their high potentials for plant regeneration.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(4), 435-440, 2007-09-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  20件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021912021
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8905183
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ