Isolation of novel gain- and loss-of-function alleles of the circadian clock gene LATE ELONGATED HYPOCOTYL (LHY) in Arabidopsis
In <i>Arabidopsis</i>, two myb-related proteins, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), play key roles in the control of circadian rhythms. Photoperiodic flowering and elongation of hypocotyls are regulated by a circadian clock. Here, we report the isolation and characterization of a novel deletion allele (<i>lhy-14</i>) and a gain-of-function allele (<i>lhy</i>-<i>2</i>) of <i>lhy</i>. Based on long hypocotyl and late flowering phenotypes under long days, <i>lhy-2</i> was isolated as an intragenic suppressor of <i>lhy-12</i>, one of the loss-of-function alleles of <i>lhy</i>. Although the <i>lhy-12</i> mRNA has a 19-bp truncation, we found that this was not caused by a deletion in the <i>LHY</i> but by a point mutation. We propose a model explaining the loss-of-function of <i>LHY</i> in <i>lhy-12</i> and the partial suppression of the <i>lhy-12</i> phenotype by <i>lhy-2</i>, by the abnormal splicing of <i>LHY</i> mRNA.
- Plant biotechnology
Plant biotechnology 24(5), 457-465, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology