Expression patterns of Arabidopsis ERF VIII-b subgroup genes during in vitro shoot regeneration and effects of their overexpression on shoot regeneration efficiency
The <i>Arabidopsis ESR1</i>, which encodes a member of the ERF family, is thought to be a key gene for commitment to shoot differentiation in tissue culture. The <i>Arabidopsis</i> genome has more than a hundred genes encoding the ERF family and <i>ESR1</i> belongs to the ERF VIII-b subgroup. We examined <i>ESR1</i> and 5 genes structurally similar to <i>ESR1</i> (<i>ESR2</i>, <i>ESL1</i>, <i>ESL2</i>, <i>ESL3</i> and <i>LEP</i>) on their expression patterns during <i>in vitro</i> shoot regeneration. All these genes' transcript levels increased 1–2 days after shoot induction by incubation on shoot-inducing medium and then decayed by day 7. These genes' overexpression demonstrated that <i>ESR1</i> and <i>ESR2</i> clearly enhanced shoot regeneration when overexpressed, but other genes did not. These results suggest that all 6 examined genes may be involved in early events of shoot regeneration, although only <i>ESR1</i> and <i>ESR2</i> enhanced shoot regeneration by their overexpression.
- Plant biotechnology
Plant biotechnology 24(5), 481-486, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology