Calcium-dependent protein phosphorylation in hairy roots of Daucus carota

この論文にアクセスする

この論文をさがす

著者

抄録

<i>In vitro</i> protein phosphorylation in soluble fractions (85,000×<i>g</i> supernatant) prepared from carrot hairy roots, which were induced by inoculation with <i>Agrobacterium rhizogenes</i> harboring the Ri plasmid, was analyzed by SDS-PAGE and autoradiography. The autoradiograms indicated that at least four proteins, with molecular masses of 65, 57.5, 52, and 38 kDa, were phosphorylated. The presence of Ca<sup>2+</sup> enhanced the phosphorylation of the 65, 57.5, and 52 kDa proteins, but the presence of both Ca<sup>2+</sup> and calmodulin did not enhance phosphorylation as much as Ca<sup>2+</sup> alone. W-7 strongly inhibited the phosphorylation of the 65, 57.5, and 52 kDa proteins while W-5 mildly inhibited the phosphorylation of these proteins. The extent of phosphorylation of these proteins and the growth rate of the hairy roots became higher with increasing incubation temperature. W-7 had a considerable inhibitory effect on the growth of hairy roots, W-5 had a weak inhibitory effect on the growth of the roots, and a high concentration of TMB-8 inhibited growth. These results suggest that the 65, 57.5, and 52 kDa proteins are phosphorylated by CDPK and that the phosphorylation is connected with the increase in growth of carrot hairy roots.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(5), 487-493, 2007-12-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  44件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021912236
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9302434
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ