Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin

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Some polyacylated anthocyanins, anthocyanins containing two or more aromatic acyl groups, are blue in color within a wide range of pH values, including acidic and neutral conditions even in the absence of any co-pigments or metal ions. We engineered a mutant of a malonyl-CoA:anthocyanin 5-<i>O</i>-glucoside-6″′-<i>O</i>-malonyltransferase of <i>Salvia splendens</i> (Ss5MaT1), Ss5AT306, which has an acquired specificity for hydroxycinnamoyl-CoA and is able to produce novel polyacylated anthocyanins. Ss5AT306 showed 6″′-<i>O</i>-hydroxycinnamoyltransferase activity toward some anthocyanins in addition to the 6″′-<i>O</i>-malonyltransferase activity retained (relative activities for acyl transfer to shisonin: malonyl-CoA, 100%; <i>p</i>-coumaroyl-CoA, 132%; caffeoyl-CoA, 103%). This alteration of acyl-donor specificity was achieved by the substitutions of only three contiguous amino acid residues, Val39-Arg40-Arg41, to the corresponding residues of anthocyanin aromatic acyltransferases, Met-Leu-Gln, suggesting that these amino acid residues are key residues governing the malonyl-CoA specificity of Ss5MaT1. Through the use of Ss5AT306, a novel polyacylated anthocyanin, <i>p</i>-coumaroylshisonin, with one aromatic acyl group in each of the 3-<i>O</i>-glucosyl and 5-<i>O</i>-glucosyl moieties of its structure, was produced and characterized <i>in vitro</i>. <i>p</i>-Coumaroylshisonin was bluer in color and displayed stronger color intensity than did shisonin, implying that accumulation of polyacylated anthocyanins such as <i>p</i>-coumaroylshisonin cause the modulation of flower colors.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 24(5), 495-501, 2007-12-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10021912281
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9302441
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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