Characterization of three halide methyltransferases in Arabidopsis thaliana

この論文にアクセスする

この論文をさがす

著者

抄録

Methyl chloride and methyl bromide, which contribute to the destruction of the stratospheric ozone layer, are mainly emitted from natural sources. It was recently reported that tropical and subtropical plants were the largest sources of methyl chloride. Furthermore, the involvement of the gene <i>HARMLESS TO OZONE LAYER (HOL)</i> in methyl halide emissions from <i>Arabidopsis thaliana</i> was demonstrated. However, neither the physiological significance of the methyl chloride emission nor the biochemical properties of HOL, denoted as AtHOL1 in our study, have been reported yet. We identified two additional isoforms-<i>AtHOL2</i> and <i>AtHOL3</i>-from <i>Arabidopsis</i> and characterized them together with <i>AtHOL1</i>. <i>AtHOL1</i> was ubiquitously expressed during development, and its expression level was the highest among the three. The phylogenetic tree suggested that AtHOL1 homologous proteins were distributed throughout the plant kingdom. Biochemical analyses showed that the three recombinant AtHOL proteins were functional and had distinct levels of the S-adenosyl-L-methionine-dependent methyltransferase activities. Although a study of <i>AtHOL1</i>-disrupted mutants had shown that <i>AtHOL1</i> primarily controlled the production of methyl halide, our study suggested that the activation of <i>AtHOL2</i> and <i>AtHOL3</i> genes also contribute to the methyl halide emissions from <i>Arabidopsis</i>.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 24(5), 503-506, 2007-12-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  17件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  2件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021912308
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    9302514
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ