Agrobacterium-mediated transformation of indica rice under Acetosyringone-free conditions
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The possibility of developing transgenic <i>indica</i> rices through <i>Agrobacterium</i>-mediated transformation in the absence of acetosyringone at bacterial preinduction or co-cultivation or both stages was assessed. Four-week-old, scutellum derived calluses of <i>indica</i> rice (<i>Oryza sativa</i> L. cv. 'Pusa Basmati1') were co-cultivated with <i>A. tumefaciens</i> strain LBA4404 (pSB1), harboring the binary vector pCAMBIA 1301 with the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes in the T-DNA region. Addition of acetosyringone (AS) to both preinduction medium (PIM) and co-cultivation medium (CCM) induced higher levels of transient GUS expression than that obtained with the addition of AS to either of the stages. Addition of only sucrose to both preinduction and co-cultivation media yielded transient expression levels similar to those obtained by the addition of AS. The resultant fertile plants were stable transformants as revealed by GUS histochemical assay and PCR analysis for the GUS and HPT genes. Thus, phenolics like AS may not be essential for induction of <i>vir</i> genes, and development of transgenic <i>indica</i> rice is feasible under AS-free conditions.
- Plant tissue culture letters
Plant tissue culture letters 24(5), 507-511, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology