Identification and Genetic relationship among Polyscias and Schefflera (Araliaceae) using RAPD and ISSR markers
The species/varieties of <i>Polyscias</i> and <i>Schefflera</i> are important ornamental foliage pot plants. The germplasm identification and characterization is an important link between the conservation and utilization of plant genetic resources. Investigation were undertaken for identification and determination of genetic variation within 14 species/varieties of <i>Polyscias</i> and one species of <i>Schefflera elegantissima</i> under family Araliaceae through RAPD (random amplified polymorphic DNA) and ISSR (Inter Simple Sequence Repeats) markers. Genetic analysis was made by using 15 selected decamer primers and 9 selected ISSR markers. A total of 164 and 69 distinct DNA fragments ranging from 300 to 2500 bp were amplified by using selected random RAPD and ISSR primers respectively. The genetic similarity was evaluated on the basis of presence or absence of bands. The cluster analysis was made by using similarity coefficient. The cluster analysis indicated that the 14 varieties/species of <i>Polyscias</i> formed one major cluster and <i>Schefflera elegantissima</i> forming another major cluster. There were distant variation among the genus or species and close variation was obtained among the varieties of <i>Polyscias</i>. The correlation matrix indicates that there was significant correlation between ISSR and RAPD markers. Thus, these markers have the potential for identification of species/varieties and variation within the varieties. This is also helpful in breeding programs as well as a major input into conservation biology of foliage crop.
- Plant biotechnology
Plant biotechnology 24(5), 519-525, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology