Micropropagation and in vitro flowering in Pentanema indicum Ling
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In this study we have investigated micropropagation and <i>in vitro</i> flowering for a medicinally important plant <i>Pentanema indicum</i>. Maximum callus proliferation was obtained on MS medium supplemented with 2.0 mg l<sup>−1</sup> BAP and 1.0 mg l<sup>−1</sup> IBA. The best shoot regeneration (19±1.0) was achieved in five weeks when callus was cultured on MS medium amended with 4.0 mg l<sup>−1</sup> BAP and 1.0 mg l<sup>−1</sup> IAA. Direct multiple shoot initiation was also obtained from shoot tip and nodal explants in the presence of BAP and IAA. Addition of adenine sulfate (1.0 mg l<sup>−1</sup>) to the regeneration medium increased the shoot multiplication. Regenerated shoots rooted best on MS medium containing 2.0 mg l<sup>−1</sup> IBA. Multiple shoots regenerated from callus, shoot tip and nodal explants flowered (90%) <i>in vitro</i> on a MS medium fortified with 2.0 mg l<sup>−1</sup> IBA. Plantlets were successfully acclimatized in a soil condition and the survival rate was 96%.
- Plant tissue culture letters
Plant tissue culture letters 24(5), 527-532, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology