Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka
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A soybean variety, Kariyutaka was found to form much more precocious flower buds than those of the other six genotypes used in this study when <i>in vitro</i> cultured at 26°C with 16/8 h light/dark regime (fluorescent tubes at an intensity of about 100 μmols s<sup>−1</sup> m<sup>−2</sup>). In addition, it was a suitable genotype for soybean transformation because it was highly sensitive to <i>Agrobacterium</i> and it exhibited excellent shoot regeneration. Its transformation frequency (about 1%) was comparable to that of a variety Thorne, which is known as one of the suitable cultivars for <i>Agrobacterium</i>-mediated transformation. Because transgenic shoots of Kariyutaka formed <i>in vitro</i> precocious flower buds, T<sub>1</sub> seeds were obtained in only six months after co-cultivation with <i>Agrobacterium</i>. A few copy numbers of transgenes were inheritable to T<sub>1</sub> generations. T<sub>1</sub> plants also produced seed within 2–3 months when grown at the same regime as <i>in vitro</i> culture. Hence <i>Agrobacterium</i>-mediated transformation using Kariyutaka can be recommended as a model method for the rapid development of transgenic soybean lines.
- Plant tissue culture letters
Plant tissue culture letters 24(5), 533-536, 2007-12-01
Japanese Society for Plant Cell and Molecular Biology