Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka
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- Sato Hiroshi
- Graduate School of Agriculture, Hokkaido University
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- Yamada Tetsuya
- Graduate School of Agriculture, Hokkaido University
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- Kita Yohichi
- Graduate School of Agriculture, Hokkaido University
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- Ishimoto Masao
- National Agricultural Research Center for Hokkaido Region
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- Kitamura Keisuke
- Graduate School of Agriculture, Hokkaido University
Bibliographic Information
- Other Title
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- Transgenic note: Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka
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Abstract
A soybean variety, Kariyutaka was found to form much more precocious flower buds than those of the other six genotypes used in this study when in vitro cultured at 26°C with 16/8 h light/dark regime (fluorescent tubes at an intensity of about 100 μmols s−1 m−2). In addition, it was a suitable genotype for soybean transformation because it was highly sensitive to Agrobacterium and it exhibited excellent shoot regeneration. Its transformation frequency (about 1%) was comparable to that of a variety Thorne, which is known as one of the suitable cultivars for Agrobacterium-mediated transformation. Because transgenic shoots of Kariyutaka formed in vitro precocious flower buds, T1 seeds were obtained in only six months after co-cultivation with Agrobacterium. A few copy numbers of transgenes were inheritable to T1 generations. T1 plants also produced seed within 2–3 months when grown at the same regime as in vitro culture. Hence Agrobacterium-mediated transformation using Kariyutaka can be recommended as a model method for the rapid development of transgenic soybean lines.
Journal
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- Plant Biotechnology
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Plant Biotechnology 24 (5), 533-536, 2007
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679303368576
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- NII Article ID
- 10021912409
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD1cXhtFGqur8%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 9303070
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed