Flower color modification of gentian plants by RNAi-mediated gene silencing

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RNA interference (RNAi) is an efficient and powerful technique for gene silencing compared with antisense and sense suppression. Here we report adaptation of RNAi technology to modify flower colors in gentian, targeted for suppression of three anthocyanin biosynthetic genes; chalcone synthase (<i>CHS</i>), anthocyanidin synthase (<i>ANS</i>) and flavonoid 3′,5′-hydroxylase (<i>F3′5′H</i>). The petals of transgenic gentian plants with a suppressed <i>CHS</i> gene exhibited pure white to pale-blue color, while those with a suppressed <i>ANS</i> gene showed only pale-blue. The suppression of the <i>F3′5′H</i> gene decreased delphinidin derivatives and increased cyanidin derivatives, and led to magenta flower colors. Northern blot analyses confirmed that all transgenic gentian plants showing typical phenotypes had strongly suppressed transcriptions of the targeted genes, corresponding with a change in anthocyanin accumulation and composition in their petals. Some rolCpro-CHSir transgenic gentians exhibited bicolor phenotypes with reduced anthocyanin accumulation along the vascular bundles. These data demonstrated that the suppression of anthocyanin biosynthetic genes by RNAi was successfully applied to gentian plants to change flower color, and this could be useful for designing novel flower color and patterns. Transgenic gentian plants produced in this study might be utilized as elite materials in the breeding of gentian plants in the near future.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(1), 61-68, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021912635
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9423468
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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