Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
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We investigated the efficiency of the 5′-untranslated region (UTR) of the tobacco <i>alcohol dehydrogenase</i> gene (<i>NtADH</i>-5′UTR) as a translational enhancer in chrysanthemum (<i>Chrysanthemum morifolium</i>) and torenia (<i>Torenia fournieri</i>). Three constructs were introduced: control, "ADH−," cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::β-<i>glucuronidase</i> (<i>GUS</i>); <i>NtADH</i>-5′UTR with a spacer, "ADH+S," CaMV 35S promoter::<i>NtADH</i>-5′UTR::25-bp spacer::<i>GUS</i>; and <i>NtADH</i>-5′UTR with no spacer, "ADH+," CaMV 35S promoter::<i>NtADH</i>-5′UTR::<i>GUS</i>. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH− plants. <i>NtADH</i>-5′UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH−), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH−), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. <i>NtADH</i>-5′UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.
- Plant tissue culture letters
Plant tissue culture letters 25(1), 69-75, 2008-03-01
Japanese Society for Plant Cell and Molecular Biology