Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene

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We investigated the efficiency of the 5′-untranslated region (UTR) of the tobacco <i>alcohol dehydrogenase</i> gene (<i>NtADH</i>-5′UTR) as a translational enhancer in chrysanthemum (<i>Chrysanthemum morifolium</i>) and torenia (<i>Torenia fournieri</i>). Three constructs were introduced: control, "ADH−," cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::β-<i>glucuronidase</i> (<i>GUS</i>); <i>NtADH</i>-5′UTR with a spacer, "ADH+S," CaMV 35S promoter::<i>NtADH</i>-5′UTR::25-bp spacer::<i>GUS</i>; and <i>NtADH</i>-5′UTR with no spacer, "ADH+," CaMV 35S promoter::<i>NtADH</i>-5′UTR::<i>GUS</i>. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH− plants. <i>NtADH</i>-5′UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH−), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH−), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. <i>NtADH</i>-5′UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.

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  • Plant biotechnology  

    Plant biotechnology 25(1), 69-75, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021912665
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9423484
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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