Improvement of regeneration and transformation systems for Cyclamen persicum using somatic embryo culture

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<i>Cyclamen persicum</i> is one of the most important pot plants in the world. We developed an effective method for somatic embryo-mediated plant regeneration and genetic transformation using <i>Agrobacterium</i>, with somatic embryos as the source of plant material. Numerous somatic embryos were formed from the leaf segments of <i>Cyclamen</i> cv. "Fragrance Mini" 16 weeks after initiation on a 2,4-D- and kinetin-containing medium. The maximum regeneration frequency from the somatic embryos was achieved using a regeneration medium containing 0.1 mg l<sup>−1</sup> benzylaminopurine, 0.01 mg l<sup>−1</sup> α-naphthaleneacetic acid, and 0.2 mg l<sup>−1</sup> gibberellin. After co-cultivating the somatic embryos with <i>Agrobacterium</i> strain LBA4404 (the plasmid vector pIG121Hm) that harbors genes for hygromycin phosphotransferase, neomycin phosphotransferase, and β-glucuronidase, hygromycin-resistant clones were selected on a medium containing 5 mg l<sup>−1</sup> hygromycin. Hygromycin-resistant plantlets were regenerated from 14 hygromycin-resistant embryos. Using this transformation system, 74 independent plants were obtained from 1-ml packed cell volume, approximately 2,000 embryos.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(1), 77-80, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021912692
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    9423490
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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