High-efficiency improvement of transgenic torenia flowers by ion beam irradiation

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To shorten the time required for breeding and optimize the risk-cost/benefit ratio of genetically modified ornamental plants, we applied heavy-ion beam irradiation to wild-type and genetically modified torenia (<i>Torenia fournieri</i> Lind. CV. 'Crown Violet' plants in which petal color and pattern had been modified by controlling two anthocyanin biosynthesis-related genes encoding chalcone synthase (<i>CHS</i>) and dihydroflavonol-4-reductase (<i>DFR</i>). Ion beams of <sup>12</sup>C<sup>6+</sup> and <sup>20</sup>Ne<sup>10+</sup> were applied to 11,500 leaf disks from wild type and five transgenic lines, and over 3,200 regenerated flowering plants were then investigated for visible phenotypes. The mutation rate after whole irradiation averaged 10.4%, and the maximum rate in the initial screening was 44.2% (<sup>20</sup>Ne, 30 Gy). Mutant phenotypes were observed mainly in flowers and showed wide variation in color and shape. Mutation efficiencies for petal color and coloration pattern were higher in transgenic plants than in wild-type plants, while those for petal shape and corolla divergence were almost equivalent in the two plant groups. Mutation spectrums in petal color in transformant-based mutants were obviously wider than those in wild-type plants. Among these mutants, a class B gene-deficient mutant was investigated as a model case for further study to facilitate the control of flower phenotype. Expression of the <i>TfGLO</i> gene was found to be repressed in this line, probably due to dysfunctioning of the upstream signaling. We propose that the combination of genetic engineering and ion beam irradiation greatly facilitates improvement of agrobiological and commercial traits within a short period. We also discuss characteristic changes observed at high frequency in torenia flowers and the mutant-based approach to the identification of useful genes.

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  • Plant biotechnology  

    Plant biotechnology 25(1), 81-89, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021912702
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9423504
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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