Heavy-ion beam-induced sterile mutants of verbena (Verbena*hybrida) with an improved flowering habit
-
- Kanaya Takeshi
- R&D Department, Suntory Flowers Ltd.
-
- Saito Hiroyuki
- Radiation Biology Team, RIKEN Nishina Center
-
- Hayashi Yoriko
- Radiation Biology Team, RIKEN Nishina Center
-
- Fukunishi Nobuhisa
- Radiation Biology Team, RIKEN Nishina Center
-
- Ryuto Hiromichi
- Radiation Biology Team, RIKEN Nishina Center
-
- Miyazaki Kiyoshi
- R&D Department, Suntory Flowers Ltd.
-
- Kusumi Takaaki
- R&D Department, Suntory Flowers Ltd.
-
- Abe Tomoko
- Radiation Biology Team, RIKEN Nishina Center
-
- Suzuki Ken-ichi
- Production Department, Suntory Flowers Ltd.
Bibliographic Information
- Other Title
-
- Heavy-ion beam-induced sterile mutants of verbena (Verbena×hybrida) with an improved flowering habit
Search this article
Abstract
Sterile mutants of Verbena×hybrida were isolated at high frequency from nodal cultures of developed plants irradiated with heavy-ion beams. Sixty four in vitro-cultured nodes of fertile cultivars ‘Temari Sakura’ (FS), ‘Temari Coral Pink’ (FC) and ‘Temari White’ (FW) were irradiated with 1 to 10Gy of 14N-ion beam (1890MeV). Lateral shoot development of FS, FC and FW was not affected by irradiation with up to 10Gy. After open-pollination, shoots with inflorescence forming unenlarged ovaries were selected and propagated several times by cutting. Shoots were grown to flowering and the selection process for isolating stable sterile mutants was carried out by the same method. Finally, one mutant out of 104 FS lateral shoots (5Gy), one mutant out of 115 FC shoots (5Gy) and 3 mutants out of 108 FC shoots (10Gy) were successfully isolated. With the exception of sterility all these mutants showed normal morphology. Two sterile mutants SS and SC, which were isolated from 5Gy-irradiated FS and FC, respectively, were characterized by their flowering habits. These two mutants grew well, had a larger number of inflorescences, and a better longevity compared with their parental cultivars. These results show that heavy-ion beam irradiation is an excellent tool for isolating sterile mutants without alterations in others important traits at a high frequency. In addition, the characterization of SS and SC indicated that they have different sterile phenotypes: male and female gametes of SS are non-functional, in contrast, SC exhibits self-incompatibility, which results in mutants unable to produce seeds.
Journal
-
- Plant Biotechnology
-
Plant Biotechnology 25 (1), 91-96, 2008
Japanese Society for Plant Biotechnology
- Tweet
Details
-
- CRID
- 1390001204327928448
-
- NII Article ID
- 10021912731
-
- NII Book ID
- AA11250821
-
- ISSN
- 13476114
- 13424580
- http://id.crossref.org/issn/13424580
-
- NDL BIB ID
- 9423519
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed