Characterization of SBZ1, a soybean bZIP protein that binds to the chalcone synthase gene promoter

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Several plant basic leucine zipper (bZIP) proteins have been shown to play a role in chalcone synthase (<i>CHS</i>) gene expression, and some are regulated by phosphorylation/dephosphorylation. We isolated <i>SBZ1</i> (Soybean bZIP protein 1) and showed that the recombinant protein binds <i>in vitro</i> to the 5′ region of soybean <i>CHS1</i>, at a sequence that confers the elicitor-inducible expression of <i>CHS</i> genes. The deduced amino acid sequence of SBZ1 has features characteristic of bZIP transcription factors, including a highly basic putative DNA-binding domain containing a nuclear localization sequence, as well as four domains designated D1–D4 that are highly conserved among the subfamily of bZIP factors, which includes tobacco BZI-1 and parsley CPRF2. The presence of these regions indicates that SBZ1 is a CPRF2-related bZIP transcription factor. The protein kinase inhibitor K252a blocks <i>CHS</i> induction in elicited soybean cells, suggesting that protein phosphorylation is involved in induction of the <i>CHS</i> signal pathway. Phosphorylation assays indicated that SBZ1 is phosphorylated <i>in vitro</i> in a soybean cell extract, and that this phosphorylation depends on Ca<sup>2+</sup>. Furthermore, recombinant soybean CDPK and the α subunit of CKII phosphorylate SBZ1 <i>in vitro</i>. However, unlike other related bZIP proteins, phosphorylation had no effect on either the DNA-binding activity of SBZ1. Therefore, we conclude that SBZ1 is regulated by phosphorylation, but in a different manner than are related bZIP factors.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(2), 131-140, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021912903
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9422917
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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