Overexpression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco

• Uchida Akio

  Graduate School of Agricultural Science, Nagoya University SCIVAX Co.

• Hibino Takashi

  Graduate School of Environmental and Human Sciences, Meijo University

• Shimada Takiko

  Research Institute of Agricultural Resources, Ishikawa Agricultural Collage

• Saigusa Masahiko

  Graduate School of Agricultural Science, Tohoku University

• Takabe Tetsuko

  Graduate School of Agricultural Science, Nagoya University

• Araki Etsuko

  Research Institute, Meijo University

• Kajita Hiroshi

  SCIVAX Co.

• Takabe Teruhiro

  Graduate School of Environmental and Human Sciences, Meijo University Research Institute, Meijo University

Abstract

The DnaK/Hsp70 family is a molecular chaperone that binds non-native states of other proteins, and concerns to various physiological processes in the bacterial, plant and animal cells. Transgenic tobacco plants expressing a molecular chaperone DnaK from a halotolerant cyanobacterium Aphanothece halophytica show enhanced seed yields as well as enhanced tolerances for salt and heat stresses. High-temperature treatment during the reproductive stage decreased total dry weight of seeds in both transgenic and wild-type tobacco, but more severely in the wild-type. Transgenic tobacco plants exhibited higher activities of ascorbate peroxidase and catalase than wild-type plants. Similar results were obtained for salt stress during the reproductive stage. Transgenic rice plants expressing ApDnaK was also constructed. Transgenic rice plants exhibited the enhanced activities for Calvin-cycle enzymes, and showed faster growth and higher seed yield compared with the wild-type rice under normal growth conditions. Transgenic rice plants also showed enhanced tolerance for high temperature and salt stress compared with the wild-type rice. These results suggest a relation of increased folding activity with enhanced stress tolerance, increased seed yield, and total plant biomass.

Journal

• Plant Biotechnology

  Plant Biotechnology 25 (2), 141-150, 2008

  Japanese Society for Plant Biotechnology

Keywords

• <i>Aphanothece halophytica</i>
• chaperone
• DnaK
• high temperature stress
• rice
• salt tolerance
• seed yield

Details

• CRID: 1390282679304400512
• NII Article ID: 10021912952
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.25.141
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 9422926
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9422926
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed