Stimulation effects of salts on growth in suspension culture of a mangrove plant, Sonneratia alba, compared with another mangrove, Bruguiera sexangula and non-mangrove tobacco BY-2 cells
Effects of a wide range of concentrations of four salts (NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>) on cell proliferation were investigated in suspension culture of a mangrove plant, <i>Sonneratia alba</i>. The results were compared to those obtained with suspension culture of another mangrove species, <i>Bruguiera sexangula</i> and non-mangrove tobacco BY-2 cells. Effects of salts on suspension cells were determined with both large scale (LS) method using a 100 ml culture flask, and/or a small scale (SS) method using a 24-well culture plate. Settled cell volume (SCV), packed cell volume (PCV), wet weight (WW) and dry weight (DW) were measured in LS method and PCV was in SS method. High concentrations of NaCl (25–100 mM) and MgCl<sub>2</sub> (25 mM) showed strong stimulatory effects on growth in <i>S. alba</i> suspension culture, and low concentrations of KCl, MgCl<sub>2</sub> and CaCl<sub>2</sub> (10 mM) also showed similar stimulatory effects. In contrast, a remarkable stimulatory effect was not observed in <i>B. sexangula</i> and tobacco BY-2 cells. Cationic ions are effective for the growth of suspension cells in both mangrove species <i>S. alba</i> and <i>B. sexangula</i>. The SS method using multi-well culture plate and micro-tube measurement method developed in this report, can be applied for analysis of factors for stress tolerance.
- Plant biotechnology
Plant biotechnology 25(2), 151-155, 2008-03-01
Japanese Society for Plant Cell and Molecular Biology