Identification of DNA polymorphisms in Angelica acutiloba

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<i>Angelica acutiloba</i> and related species have been known for their analgesic and sedative effects, and are widely used as the key ingredient in Japanese traditional Kampo medicine. Among various varieties, <i>Angelica acutiloba</i> var. <i>acutiloba</i> has long been grown in the Obuka area of Nara prefecture, Japan, and has been evaluated as an excellent variety. Recent cultivation of this and other varieties of <i>Angelica</i> in various regions of East Asia prompted us to identify DNA polymorphisms that distinguish the <i>acutiloba</i> variety from others. Random Amplified Polymorphic DNA analysis and sequencing selected genomic regions among <i>Angelica</i> plants of different origins identified several nucleotide changes among the varieties tested, which enabled us to distinguish the <i>acutiloba</i> variety from others. We also report an improved protocol for genomic DNA extraction from dried root samples of <i>Angelica</i>.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(2), 157-163, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021913000
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    9422944
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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