Identification of DNA polymorphisms in Angelica acutiloba
<i>Angelica acutiloba</i> and related species have been known for their analgesic and sedative effects, and are widely used as the key ingredient in Japanese traditional Kampo medicine. Among various varieties, <i>Angelica acutiloba</i> var. <i>acutiloba</i> has long been grown in the Obuka area of Nara prefecture, Japan, and has been evaluated as an excellent variety. Recent cultivation of this and other varieties of <i>Angelica</i> in various regions of East Asia prompted us to identify DNA polymorphisms that distinguish the <i>acutiloba</i> variety from others. Random Amplified Polymorphic DNA analysis and sequencing selected genomic regions among <i>Angelica</i> plants of different origins identified several nucleotide changes among the varieties tested, which enabled us to distinguish the <i>acutiloba</i> variety from others. We also report an improved protocol for genomic DNA extraction from dried root samples of <i>Angelica</i>.
- Plant biotechnology
Plant biotechnology 25(2), 157-163, 2008-03-01
Japanese Society for Plant Cell and Molecular Biology