The 5'-end expressed sequence tags of Lotus japonicus

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For a global analysis of gene expression during nodule tissue formation in a model legume, <i>Lotus japonicus</i>, a cDNA array was constructed using 18,144 non-redundant clone set deduced from 3′-end expressed sequence tags (EST, Kouchi et al. 2004). In the current study, to obtain peptide coding information from 5′-ends of clone, we generated 7,320 5′ EST sequences. BLAST search against a protein database indicated that 65.5% of the ESTs showed significant similarity to registered sequences. We identified numerous of nodule-enhanced genes, including many involved in diverse cellular processes, suggesting that the EST resources generated in this study will be a useful tool for the identification of genes related to nodulation and symbiotic nitrogen fixation.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(2), 173-175, 2008-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021913054
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    9422964
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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