The 5'-end expressed sequence tags of Lotus japonicus

• Nomura Mika

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Tan Dao Van

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Arunothayanan Hatthaya

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Asamizu Erika

  Kazusa DNA Research Institute

• Tabata Satoshi

  Kazusa DNA Research Institute

• Kouchi Hiroshi

  National Institute of Agrobiological Sciences

• Tajima Shigeyuki

  Department of Life Science, Faculty of Agriculture, Kagawa University

Abstract

For a global analysis of gene expression during nodule tissue formation in a model legume, Lotus japonicus, a cDNA array was constructed using 18,144 non-redundant clone set deduced from 3′-end expressed sequence tags (EST, Kouchi et al. 2004). In the current study, to obtain peptide coding information from 5′-ends of clone, we generated 7,320 5′ EST sequences. BLAST search against a protein database indicated that 65.5% of the ESTs showed significant similarity to registered sequences. We identified numerous of nodule-enhanced genes, including many involved in diverse cellular processes, suggesting that the EST resources generated in this study will be a useful tool for the identification of genes related to nodulation and symbiotic nitrogen fixation.

Journal

• Plant Biotechnology

  Plant Biotechnology 25 (2), 173-175, 2008

  Japanese Society for Plant Biotechnology

Keywords

• ESTs
• Legume
• <i>Lotus japonicus</i>
• Nitrogen fixation

Details

• CRID: 1390001204327693952
• NII Article ID: 10021913054
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.25.173
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 9422964
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9422964
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed