Characterization of PAP1-upregulated Glutathione S-transferase genes in Arabidopsis thaliana
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Glutathione <i>S</i>-transferase (GST) plays an important role in the transport and accumulation of anthocyanin and proanthocyanidin in plants. In our previous study on <i>Arabidopsis thaliana</i> overexpressing the <i>PRODUCTION OF ANTHOCYANIN PIGMENT 1</i> (<i>PAP1</i>) gene encoding an MYB transcription factor, the <i>AtGSTF5</i> and <i>AtGSTF6</i> genes encoding GST-like protein were up-regulated along with <i>TRANSPARENT TESTA 19</i> (<i>TT19</i>), which is required for the accumulation of anthocyanin and proanthocyanidin. The proteins encoded by these 3 genes showed very weak GST activities as detected by using recombinant proteins expressed in <i>Escherichia coli.</i> The anthocyanin levels were severely decreased in the <i>tt19</i> mutant but not in the <i>Atgstf6</i> mutant, suggesting that <i>TT19</i> is almost exclusively involved in anthocyanin accumulation. The results of co-expression network analysis using public transcriptome data corresponded to the proposition of the predominant role of <i>TT19</i> in anthocyanin accumulation.
- Plant tissue culture letters
Plant tissue culture letters 25(2), 191-196, 2008-03-01
Japanese Society for Plant Cell and Molecular Biology