Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)
In order to generate transgenic radish (<i>Raphanus sativus</i> L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4 mg l<sup>−1</sup> AgNO<sub>3</sub>, 5 mg l<sup>−1</sup> acetosyringone, 4 mg l<sup>−1</sup> 6-benzyladenine, and 3 mg l<sup>−1</sup> α-naphthaleneacetic acid in addition to either 10 mg l<sup>−1</sup> hygromycin or 100 mg l<sup>−1</sup> paromomycin after co-cultivation with disarmed <i>Agrobacterium tumefaciens</i> harboring a plant expression binary vector. Explants co-cultivated with <i>A. tumefaciens</i> GV3101 harboring pCAMBIA1301 and <i>A. tumefaciens</i> EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the <i>gus</i> gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the <i>gus</i> gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.
- Plant biotechnology
Plant biotechnology 25(2), 205-208, 2008-03-01
Japanese Society for Plant Cell and Molecular Biology