Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)
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- Cho Mi Ae
- Department of Oriental Pharmaceutical Development, Nambu University
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- Min Sung Ran
- Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
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- Ko Suk Min
- Eugentech Inc., Bio Venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
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- Liu Jang Ryol
- Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
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- Choi Pil Son
- Department of Oriental Pharmaceutical Development, Nambu University
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Abstract
In order to generate transgenic radish (Raphanus sativus L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4 mg l−1 AgNO3, 5 mg l−1 acetosyringone, 4 mg l−1 6-benzyladenine, and 3 mg l−1 α-naphthaleneacetic acid in addition to either 10 mg l−1 hygromycin or 100 mg l−1 paromomycin after co-cultivation with disarmed Agrobacterium tumefaciens harboring a plant expression binary vector. Explants co-cultivated with A. tumefaciens GV3101 harboring pCAMBIA1301 and A. tumefaciens EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the gus gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the gus gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.
Journal
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- Plant Biotechnology
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Plant Biotechnology 25 (2), 205-208, 2008
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304416896
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- NII Article ID
- 10021913172
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 9423073
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed