In vitro node culture of seedlings in bamboo plant, Phyllostachys meyeri McClure
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- Ogita Shinjiro
- Laboratory of Plant and Cell Engineering, Department of Biotechnology, Toyama Prefectural University
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- Kashiwagi Harutsugu
- The Fuji Bamboo Garden
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- Kato Yasuo
- Laboratory of Plant and Cell Engineering, Department of Biotechnology, Toyama Prefectural University
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Phyllostachys meyeri cultivated in the Fuji Bamboo Garden, Japan showed monocarpic mass flowering from 2004 through 2005. We first examined in vitro germination of the caryopses and found that sterilized water supplemented with 0.1% Plant Preservative MixtureTM was effective for their growth with an efficient germination rate (75%). Seedlings were transferred to a liquid modified half strength Murashige and Skoog (m1/2MS) medium for further growth and were acclimatized in soil mixture under ex vitro condition. Nodal segments which were collected from ca. 1.5 year-old seedlings and 1 year-old tissue cultured clone plants were re-sterilized and then cultured in a liquid m1/2MS medium. Shoot elongation with rooting could be seen within 8 weeks and they were successfully multiplied under ex vitro condition.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 25 (4), 381-385, 2008
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327458688
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- NII論文ID
- 10021914248
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 9558389
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可