In vitro node culture of seedlings in bamboo plant, Phyllostachys meyeri McClure
<i>Phyllostachys meyeri</i> cultivated in the Fuji Bamboo Garden, Japan showed monocarpic mass flowering from 2004 through 2005. We first examined <i>in vitro</i> germination of the caryopses and found that sterilized water supplemented with 0.1% Plant Preservative Mixture<sup>TM</sup> was effective for their growth with an efficient germination rate (75%). Seedlings were transferred to a liquid modified half strength Murashige and Skoog (m1/2MS) medium for further growth and were acclimatized in soil mixture under <i>ex vitro</i> condition. Nodal segments which were collected from <i>ca.</i> 1.5 year-old seedlings and 1 year-old tissue cultured clone plants were re-sterilized and then cultured in a liquid m1/2MS medium. Shoot elongation with rooting could be seen within 8 weeks and they were successfully multiplied under <i>ex vitro</i> condition.
- Plant biotechnology
Plant biotechnology 25(4), 381-385, 2008-09-01
Japanese Society for Plant Cell and Molecular Biology