Gene trap strategy, an effective tool for identification of novel genes expressed in anther tissues in Arabidopsis thaliana

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Analysis of GUS expression patterns was previously carried out for 20,000 gene trap lines of <i>Arabidopsis thaliana</i> generated by the Kazusa DNA Research Institute. This analysis indicated that 51 lines showed GUS expression within stamens. In this paper, we further focused on 42 of these lines in order to examine the detailed expression patterns at different anther developmental stages. Seven lines showed GUS expression exclusively in the tapetum during the early stage of anther development, thirty lines in pollen grains at the tricellular pollen stage and five lines in filaments at the tricellular pollen stage. We identified four trapped-genes by sequencing the adjacent fragments to T-DNA insertions. RT-PCR analysis confirmed that the mRNA expression patterns in these four genes were consistent with the expression pattern observed by GUS activity. Our results demonstrated that the gene trap system is an efficient strategy for identifying stamen specific genes in <i>Arabidopsis</i>.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(4), 391-395, 2008-09-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021914279
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    9558445
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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