Transcriptional regulation of an Arabidopsis gene encoding a CCT domain-containing protein during endoplasmic reticulum stress
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In eukaryotic cells, accumulation of unfolded proteins in the endoplasmic reticulum (ER) triggers the transcriptional induction of ER-resident molecular chaperones and folding enzymes to maintain cellular homeostasis, termed the ER stress response or the unfolded protein response (UPR). In this study we focused on a putative transcriptional regulator of <i>Arabidopsis thaliana</i> whose transcripts accumulate in response to the ER stress inducer tunicamycin. This gene, designated as <i>TCR1</i> (<u>t</u>unicamycin-induced <u>C</u>ONSTANS-like-<u>r</u>elated <u>1</u>), encodes a predicted protein of 195 amino acids with a plant-specific CCT (<u>C</u>ONSTANS, <u>C</u>ONSTANS-like, <u>T</u>OC1) domain considered to function in transcriptional regulation. In a dual-luciferase assay using <i>Arabidopsis</i> protoplasts, we showed that induction of the <i>TCR1</i> gene by tunicamycin is independent of a nucleotide sequence similar to the cis-element ER stress response element on its promoter. Our result further indicates that this induction could be mediated by the transcription factor AtbZIP60. This study is a first step to understand the role of TCR1 in the <i>Arabidopsis</i> ER stress response.
- Plant tissue culture letters
Plant tissue culture letters 25(4), 397-402, 2008-09-01
Japanese Society for Plant Cell and Molecular Biology