Investigation of metal exudates from tobacco glandular trichomes under heavy metal stresses using a variable pressure scanning electron microscopy system

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Tobacco (<i>Nicotiana tabacum</i>) was recently shown to detoxify heavy metals by exudation of metals as a metal-substituted calcite (calcium carbonate) through leaf trichomes. In this paper, we describe the applications of the variable-pressure scanning electron microscopy (VP-SEM) system to investigate tobacco trichomes exudates after heavy metal treatment. An energy-dispersive X-ray analysis (EDX) system fitted to VP-SEM revealed that the exudates contain amounts of heavy metals. Overexpression of cysteine synthase confers cadmium (Cd) tolerance to tobacco, and the endogenous concentration of Cd was 20% less in transgenic plants than in wild-type plants. We evaluated the numbers of trichomes on the leaf surfaces of wild-type and transgenic plants using VP-SEM. The numbers of both long and short trichomes in the transgenic plants were 25% higher than in that of wild-type plants, indicating the active excretion of Cd from trichomes in transgenic plants. The VPSEM-EDX system is a powerful tool to investigate plant epidermal structures and functions.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(4), 407-411, 2008-09-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021914328
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    9558482
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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